New Amperometric Glucose Biosensors Based On Nafion Nanofibers

Abstract

In this study, we present new amperometric glucose biosensors based on Nafion nanofibers. Nafion possesses a number of desirable properties, including stability, thermal, mechanical, physio-chemical and biocompatibility, which make it a competitive polymer for use in glucose biosensors. Nafion nanofibers were produced using a low-cost electrospinning method, and their morphology was characterized via scanning electron microscopy (SEM). The biosensors were fabricated by loading glucose oxidase (GOx) and GOx-AuNPs (10 nm) on the Nafion-Cellulose Acetate nanofibers (Nafion-CA Nfs) (461.53 ± 30.34 nm) on the surface of a platinum electrode, followed by cross-linking using glutaraldehyde. The biosensors operating at 0.6 V demonstrated lower LODs with wider linear ranges, enhanced stability, elevated sensitivity, and improved selectivity. For Pt/Nafion-CA NFs/GOx biosensor demonstrated remarkable sensitivity, with values of 68.67 \mu A.mM-1cm-² and 18.38 \mu A.mM-1.cm-², respectively, and a limit of detection (LOD) of 1.36 \mu M. Meanwhile, the Pt/Nafion-CA NFs/ GOx-AuNPs biosensor achieved sensitivities of 55.56 \mu A.mM-1.cm² and 28.49 \mu A/mM·cm², respectively. It also exhibited a broad linear range of up to 12.81 mM and an LOD of 10.8 \mu M, with no observable interference effects. The present studies highlight the potential of Nafion nanofibers as a suitable matrix for immobilizing GOx and GOx-AuNPs, thereby demonstrating their viability as interference-free, easy-to-use sensors with good sensitivity, LOD and stability for commercialization.