Plant is commonly known as one of the main dietary sources of the human being. Many plant-derived compounds have been used as drugs, either in their original or semi-synthetic form. There are also several plant extracts or “phytomedicine” in clinical trials for the treatment of various diseases. Plant-derived compounds will still be an essential aspect of the therapeutic array of medicines available to the physician, particularly with the availability of new hyphenated primary analytical methods by determining biological activities of medicinal plants. The ethanolic extracts of two creeper plants namely Asplenium trichomanes (Maidenhair spleenwort), Lagenaria siceraria (Bottle gourd), originated from different geographical part of the world were chosen to be determine its antimicrobial, antioxidants, anti-inflammatory and UV radiation absorbance activity which is mainly characterize the anticancer activity which is nowadays become the most fatal disease. Such kind of biomedical and biotechnology approaches have been aimed to eradicate naturally such illness which is the main objective of this work. The present study shows that different ethanolic leaf extracts have an important and necessary antioxidant and anti-inflammatory properties with UV absorbing activity at UV–A region. These results show that Asplenium trichomanes and Lagenaria siceraria will be potential natural sources of antioxidant and anti-inflammatory activity. They could have greater importance as therapeutic agents in preventing or treating oxidative stress and inflammation related disorders, such as cancer and other health disorders. While the negative result of the antimicrobial activity of most of both ethanolic extract is considered as a positive result depending to main purpose of future work. Because it shows that extracts will never upset the gastric flora, in which they must be applied safely away from any harmful reaction and allergic or side effects for the living organism.